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#1. mRNA Stability Assay Using transcription inhibition by ...
mRNA Stability Assay Using transcription inhibition by Actinomycin D in Mouse Pluripotent Stem Cells. Bio Protoc. 2018 Nov 5;8(21):e3072. doi: ...
#2. Post-transcriptional Stabilization of Ucp1 mRNA Protects Mice ...
After administration of a transcription inhibitor, actinomycin D, the remaining Ucp1. mRNA level was lower in iWAT of HFD-fed mice than that in.
#3. Determining mRNA half-lives on a transcriptome-wide scale
Control of mRNA stability is a key step in gene regulation. ... These include actinomycin D, a fast-acting DNA intercalator that inhibits ...
#4. (PDF) mRNA Stability Assay Using Transcription Inhibition by ...
Actinomycin D is widely used in mRNA stability assays to inhibit. the synthesis of new mRNA, allowing the assessment of mRNA decay by ...
#5. EhPgp5 mRNA Stability Is a Regulatory Event in ...
Here we evaluated the role ofEhPgp5 mRNA stability on MDR using actinomycin D.EhPgp5 mRNA from trophozoites growing without emetine had a ...
#6. Evaluating the Stability of mRNAs and Noncoding RNAs
This protocol details the transcriptional blockage of cultured cells with actinomycin D, followed by RNA isolation at different time points, ...
#7. Translation affects mRNA stability in a codon ... - eLife
To determine whether codon composition affects mRNA stability in human cells, we treated 293T, HeLa and RPE cells with Actinomycin D to ...
#8. Impact of Methods on the Measurement of mRNA Turnover
Despite the long history of mRNA half-life measurements, ... To inhibit transcription in mammalian cells, mostly actinomycin D has been used ...
#9. mRNA Degradation Plays a Significant Role in ... - ASM Journals
By blocking transcription with actinomycin D, we found that almost 40% of these genes were regulated via effects of PI 3-kinase on mRNA stability.
#10. PARP1 is a versatile factor in the regulation of mRNA stability ...
Next, WT and PARP1 KD cells were also treated with 5 μg/mL of actinomycin D, and the RNA was harvested at time points 0, 2, 6 and 24 hours post- ...
#11. mRNA degradation controls differentiation state-dependent ...
We studied mRNA stability in proliferating and differentiated myogenic cells ... Cells were treated with actinomycin D to arrest de novo RNA ...
#12. Regulation of tumour necrosis factor α mRNA stability by the ...
Mitogen-activated protein kinase p38; mRNA stability; ... cyclooxygenase-2 (Cox-2) mRNA stability has been demonstrated by actinomycin D chase experiments.
#13. Metabolic labelling of RNA uncovers the contribution ... - bioRxiv
2012), which in turn regulate mRNA stability and translation. ... Individual mRNA half-life calculation using actinomycin D.
#14. Global analysis of stress-regulated mRNA turnover by ... - PNAS
For mRNA half-life assessments, actinomycin D (2 μg/ml) was added and total RNA prepared at the times indicated. mRNA half-lives were assessed after measurement ...
#15. Altered Stability of mRNAs Associated with Glaucoma ... - IOVS
Methods.: HTM cells were treated with 300 μM hydrogen peroxide (H2O2) for 1 hour in the presence of 5 μg/mL actinomycin D and compared with untreated cells.
#16. Post-Transcriptional Deregulation of myc Genes in Lung ...
We examined whether messenger RNA (mRNA) stabilization could be ... Transcription was blocked using the drug actinomycin D (2.5 mg/ml stock ...
#17. mRNA half-life measurement in mammalian cells Georg ...
mRNA half-life can be determined using Northern blot analysis or quantitative ... 4 At t = 0, inhibit transcription by adding 5 µg/ml Actinomycin D (1:1000 ...
#18. Control of gene expression during T cell activation
Comparative analysis revealed that regulation of mRNA stability may ... were shown to be inhibited by Actinomycin D (ActD) pre-treatment of ...
#19. A Review of Methods to Monitor the Modulation of mRNA ...
that in eukaryotic cells, modulation of mrna stability by cis- ... actinomycin d or alpha-amanitin, to measure the half-life of specific mrnas.
#20. Global analysis reveals multiple pathways for unique ...
Here, we set out to identify mRNAs whose stability differs ... inhibition of transcription with actinomycin D. mRNA abundance at each time ...
#21. Hypoxia and Hypoglycemia synergistically regulate mRNA ...
Hypoxia and Hypoglycemia synergistically regulate mRNA stability ... Cells were treated with Actinomycin D for 0, 1 or 2 hours, mRNA levels of VEFG165 and ...
#22. XRN1 Stalling in the 5' UTR of Hepatitis C Virus and ... - PLOS
Differential mRNA stability is a major regulator of gene expression in cells. ... Panel A. Assessment of TUT1 mRNA stability using actinomycin D in human ...
#23. Stability of mRNA in the hyperthermophilic archaeon <i ...
mRNA is typically unstable in bacteria and stable in eukaryotes, ... Actinomycin D was the most stable and potent compound identified. A.
#24. 3179.full.pdf - The Journal of Immunology
Stability of mRNA in macrophages. RAW264.7 macrophages were stimulated with 100 ng/ml LPS for 2 h. Actinomycin D (5 mg/ml) was then added to the culture ...
#25. Wild Oat - jstor
actinomycin D resulted in the stabilization of the transcripts. Nev- ... transcription control, especially the stability of mRNAs. Control of mRNA stability ...
#26. Posttranscriptional Regulation of Endothelial Nitric Oxide ...
The half-life of eNOS mRNA, measured after actinomycin D transcriptional arrest ... Nuclear run-on assays and studies of mRNA stability were used to examine ...
#27. MIT Open Access Articles 3' UTR-isoform choice has limited ...
slightly but significantly lower mRNA stability and greater translational efficiency ... using actinomycin D and microarrays in mouse ES cells (rp = 0.68).
#28. Stability of Cytoplasmic Messenger RNA in HeLa Cells
of the quantitative behavior and synthetic capacity of cyto- plasmic polysomes after further mRNA synthesis has been arrested by actinomycin D. The results ...
#29. Eukaryotic mRNA Decay: Methodologies, Pathways, and ...
d mRNA. [. ]/dt = μ mRNA. [. ] (4). Therefore, both degradation and dilution ... transcription shutoff with actinomycin D or by in vivo.
#30. CHARACTERIZATION OF C/EBPδ mRNA STABILITY
mechanism for C/EBPδ mRNA stability in G0 growth arrest under genotroxic stress ... ACT D actinomycin D. AN anisomycin. APRIL acidic protein rich in leucine.
#31. p38 mitogen‑activated protein kinase inhibits USP22 ...
To further determine whether p38 MAPK influences USP22 mRNA stability in HeLa cells, actinomycin D was used to block de novo mRNA ...
#32. Regulation of mammalian ribonucleotide reductase Ri mRNA ...
either RIBP-RNA binding or RI mRNA-stability charac- teristics. Transfectants containing RI or ... actinomycin D (Sigma) to block transcription [8,20].
#33. Effects of inhibiting transcription and protein synthesis on ...
Actinomycin D (5 μg/ml) increased both basal and insulin-stimulated (1.6 nM) leptin secretion ... underlying the alteration of mRNA stability remain yet.
#34. mRNA stability > BRIC
actinomycin D 이것을 처리 했을때 mRNA stability를 가장 많이 봣더라고요 혹시 actinomycin D를 처리하고 mRNA level을 보지 않고 protein level을 봐도 상관이 없을지 ...
#35. Neuronal Cells Display Distinct Stability Controls of Alternative ...
Interestingly, the differential mRNA stability among APA isoforms appears to ... measured by using transcriptional shutdown with Actinomycin D (Wu et al., ...
#36. Journal of Cystic Fibrosis
Decreased mRNA and protein stability of W1282X limits response ... points after actinomycin D treatment so that decay of the.
#37. Differential stability of zein mRNA in developing corn kernel
The lifetime of the zein mRNA in a developing corn (Zea mays L.) kernel under genome transcription blockade with actinomycin D (in vivo) and in a cell-free.
#38. ARE‐mRNA degradation requires the 5′–3′ decay pathway
Whereas globin mRNA lacking the ARE is stable during a 6-h period in the presence of actinomycin D, the globin-ARE mRNA is rapidly degraded (supplementary ...
#39. Actinomycin D|RNA polymerase inhibitor - APExBIO
Have been used for many years as an older chemotherapy, actinomycin D binds to double- and single-stranded DNA to inhibit DNA and RNA synthesis by binding DNA ...
#40. Stability of the mRNA encoding some pancreatic hydrolases is ...
pancreatic hydrolase mRNA stabilities in comparison with those of control animals receiving a standard diet. Actinomycin D was used to inhibit transcription ...
#41. AGO2 Mediates MYC mRNA Stability in Hepatocellular ...
AGO2 Mediates MYC mRNA Stability in Hepatocellular Carcinoma ... E, MYC mRNA transcript levels in shCtrl or shAGO2 after Actinomycin D (ActD) or 5 ...
#42. UTR regulatory elements in activity-dependent mRNA stability ...
3. Results. Transcripts regulated by synaptic activity (BiC/4-AP treatment) independently of transcription (during actinomycin D block) (figure ...
#43. Actinomycin D-based Method - Creative Biolabs
Creative Biolabs provides custom mRNA stability test service using actinomycin D-based method for our global customers.
#44. CNOT3 contributes to early B cell development by controlling ...
For the analysis of mRNA stability, sorted pro–B cells were cultured in the presence of 5 µg/ml actinomycin D. Total RNA extracted at the indicated time ...
#45. Investigating the effect of translation inhibition on cellular ...
Our findings from these actinomycin D block assays strongly suggest that mRNAs exhibit stabilization upon treatment with select protein.
#46. HuR Mediates Changes in the Stability of AChRß-Subunit ...
at the level of mRNA stability, contribute to the changes in AChR ... treated with actinomycin D (5 μg ml 1) for 2, 4, 6, or 8 h to inhibit transcription.
#47. Induction of fetal hemoglobin through enhanced translation ...
Cells were treated with Sal in combination with actinomycin D, ... Sal does not change mRNA stability, mRNA cellular localization, or total.
#48. mRNA Degradation Plays a Significant Role in the Program of ...
By blocking transcription with actinomycin D, we found that almost 40% of ... results show that PI 3-kinase regulation of mRNA stability, ...
#49. Dishevelled-KSRP complex regulates Wnt signaling through ...
To probe mRNA half-life, F9 cells were treated without or with Wnt3a followed by addition of actinomycin D (a transcription inhibitor).
#50. Protein Turnover and Protein Synthesis Following Actinomycin ...
Actinomycin -D Injection as a Function of Age in Drosophila ... The influence of aging on the stability of mRNA has been investigated in Drosophila by ...
#51. Translation Affects mRNA Stability in a Codon-Dependent ...
WEBINAR: Genome-Wide Study Reveals a Novel Regulatory Pathway: Translation Affects mRNA Stability in a Codon-Dependent MannerABSTRACT:This ...
#52. Regulation of Gene Expression - 第 116 頁 - Google 圖書結果
For example, one may find that mRNA accumulation far exceeds what can be explained ... eukaryotic RNA polymerase inhibitors, Actinomycin D and α-amanitin.
actinomycin d, mrna stability 在 Translation Affects mRNA Stability in a Codon-Dependent ... 的推薦與評價
WEBINAR: Genome-Wide Study Reveals a Novel Regulatory Pathway: Translation Affects mRNA Stability in a Codon-Dependent MannerABSTRACT:This ... ... <看更多>