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#1. siRNA 合成服務- 力鈞生物科技有限公司ZGENEBIO BIOTECH ...
(3)Scrambled陰性對照和選中的siRNA序列有相同的組成,但是和mRNA沒有明顯的同源性; (4)陰性對照需要確定和目的靶細胞中其它基因沒有同源性。 B.螢光標記陰性對照
#2. siRNA和shRNA:通过基因沉默抑制蛋白表达的工具 - Labome
这篇综述将重点介绍shRNA和siRNA是如何导致蛋白质表达抑制的。通过几种蛋白的活性(下面讨论),通过短反义核酸(siRNA和shRNA序列)锁定细胞mRNA,从而实现其随后 ...
#3. scramble siRNA什么东西?涉及了哪些生物技术? - 百度知道
应该是scrambled siRNA吧不知道你是什么专业的,这个要讲起来很麻烦。简单的讲,scrambled是杂乱的意思,scrambled siRNA是针对目的siRNA的一个对照, ...
#4. 小干擾RNA(siRNA):發現,結構 - 中文百科全書
目前已知siRNA主要參與RNA干擾(RNAi)現象,以帶有專一性的方式調節基因的表達。此外,也參與一些與RNAi相關的反應 ... 陰性對照(scrambled siRNA)的導入(左)對GAPDH.
#5. 微小核醣核酸(MicroRNA)藥品臨床前藥毒理法規研究
寡核苷酸類藥品如siRNA,有些特殊的試驗可以減免、延後執行、免除或增加。 ... 寡核苷酸序列之製劑配方進行測試,這個序列有時也被稱為雜燴的(scrambled).
此外,您还可使用BLOCK-iT™ RNAi Designer选择任何Stealth RNAi™ siRNA序列的scrambled(序列打乱的)对照。我们的RNAi载体克隆试剂盒之中还随附一种阴性对照。
#7. 【ViralEntry™病毒轉導增強劑】 Primary T-Cell 淋巴細胞高效 ...
HEK293T, HeLa, K562, Primary Human T Cells, and Primary Swine Spleen Macrophages were transduced with Scrambled siRNA GFP Lentivirus (Cat. No.
#8. 利用TSP-1核糖核酸干擾素增加neuDNA疫苗療效
為了更加屏除U6 promoter所帶來的免疫影響,我們亦利用TSP-1 scramble siRNA當作控制組別去更專一性地証實這個假說。結果也發現scramble siRNA可以reverse TSP-1 siRNA ...
#9. 盟基生物科技股份有限公司
盟基生物科技的專業服務團隊客製化抗體提供了研究人員完整的銷售前與銷售後的技術資源,讓您於研究上無後顧之憂。客製化抗體推動蛋白質分析鑑定技術之快速發展。
#10. 缺氧誘導因子HIF1α與叉頭框轉錄因子FOXA2 共定位於
中文 題目:在人類支氣管上皮細胞中,缺氧誘導因子HIF1α與叉頭框轉錄因子FOXA2 共定位於 ... The HBECs were either transfected with a scrambled siRNA or.
#11. RNA干擾- 維基百科,自由的百科全書
慢病毒遞送設計的shRNA和在哺乳動物細胞中的RNA干擾的機制。 ... RNA干擾(RNA interference,縮寫為RNAi)是指一種分子生物學上由雙鏈RNA誘發的基因沉默現象,其機制是通過 ...
#12. 行政院國家科學委員會補助專題研究計畫 - 國立成功大學機構典藏
中文 摘要. Annexin A2是一種鈣離子依賴性結合至細胞膜磷脂質的蛋白質,通常表達在細胞質 ... scrambled siRNA (Control siRNA-A, Cat.
#13. RNA干扰技术用于哺乳动物细胞中报告基因 - 期刊
第一部分短发夹RNA介导RNA干扰的时间剂量效应及实验控制研究(中文摘要) 在功能基因组学 ... 如1)碱基错配scrambled siRNAs及阴性对照设置;2)基本实验控制,如同时 ...
#14. 沉默干扰素诱导跨膜蛋白3可抑制肝癌细胞HepG2增殖和侵袭
A: Significantly lowered expression of IFITM3 mRNA in HepG2 cells after transfection compared with the cells transfected with scrambled siRNA and control ...
#15. Suppression of diabetic retinopathy with GLUT1 siRNA ...
The diabetic scrambled siRNA and GLUT1 siRNA exhibited higher glucose concentrations than scrambled siRNA, but GLUT1 siRNA ... 中文翻译: ...
#16. VEGF siRNA delivered by polycation liposome-encapsulated ...
VEGF siRNA mediated by PLCP can reduce 60%–80% VEGF expression in vitro, ... Saline, PLCP, and PLCP with either the scrambled siRNA or the ...
#17. 特异性小干扰RNA下调中期因子基因表达对膀胱癌细胞化疗敏感 ...
摘 要:, 目的观察中期因子(MK)基因小干扰RNA(siRNA)对膀胱癌细胞化疗药物敏感性的影响 ... After transfected with MK siRNA or scrambled siRNA for different time, ...
#18. MISSION® siRNA Universal Negative Control #1 | Sigma-Aldrich
MISSION siRNA Universal Negative Control #1; MISSION siRNA Universal Negative Control #1 has been used as a negative control for siRNA transfections.
#19. Control shRNA Plasmid-A | SCBT - Santa Cruz Biotechnology
Control shRNA Plasmid-A encodes of a scrambled shRNA sequence suitable as a negative control for experiments using targeted shRNA Plasmid DNA transfection.
#20. 香蕉你的芭樂意思
... 饭塞人——讨厌人的意思; scramble siRNA什么东西? ... 中文名. 第一个场景,题主所说的Python中的“实例”,就是面向对象的实例。
#21. 重组Anti-Senataxin抗体[EPR24137-74] (ab300439) - 试剂盒
Negative control: HeLa transfected with scrambled siRNA control. Flow Cyt (Intra): HeLa cells. 常规说明. This product is a recombinant monoclonal antibody, ...
#22. DsiRNAs and TriFECTa RNAi Kits | IDT
Universal negative controls (non-targeting and scrambled DsiRNAs): The Negative Control DsiRNA is a non-targeting DsiRNA that will not interact with any ...
#23. Silencing A7-nAChR levels increases the sensitivity of gastric ...
A7-nAChR expression was silenced by small interfering RNA (siRNA) ... to ixabepilone administration than scrambled control AGS cells.
#24. GAPDH - 快懂百科
阴性对照(scrambled siRNA)的导入(左)对GAPDH蛋白的表达没有影响,不过若转入一个以GAPDH(右)为靶标的siRNA,则会导致GAPDH蛋白表达水平的急剧下降。
#25. miR-7靶向沉默EGFR抑制大鼠星形胶质细胞活化
此外,以EGFR siRNA或STAT3抑制剂S31-201处理星形胶质细胞,再与miR-7 ... 实验分为3组:对照组、Scramble siRNA组和EGFR siRNA组,对照组用培养液处理24 h,后2组 ...
#26. 2022scrambled control中文-汽車保養配件資訊,精選在PTT ...
2022scrambled control中文-汽車保養配件資訊,精選在PTT/MOBILE01討論議題,找scrambled control中文,Scrambled siRNA,scrambled control中文,shrna ...
#27. Silencer Negative Control scrambled (Scr) siRNA的翻译是 ...
Silencer Negative Control scrambled (Scr) siRNA. 5个回答. 消声阴性对照炒( SCR)的siRNA 2013-05-23 12:21:38 回答:匿名. 遏声器消极控制被扰乱的(Scr) siRNA
#28. Natural Polyphenol Inspired Polycatechols for Efficient siRNA ...
The resulting polycatechol/siRNA formulations were also functionally ... Nongene-targeted scramble siRNA (siNC) was tested in parallel ...
#29. siRNA Oligos - Genomics-online
RNA interference (RNAi) is an RNA-mediated gene silencing mechanism. As a tool for mammalian cell systems, knockdown is achieved through the delivery of a ...
#30. Age-related maculopathy susceptibility 2 participates in the ...
siRNA was used to knock down ARMS2 mRNA, and the effects of the knockdown on the ... and 98.30±0.11 in the scrambled siRNA-transfected groups, respectively.
#31. DOT1L在头颈部鳞状细胞癌中的表达及生物学意义 - 维普期刊
维普中文期刊服务平台,是重庆维普资讯有限公司标准化产品之一,本平台以《中文 ... 与Scrambled siRNA组比较,DOT1L siRNA组细胞存活率、S期、G2/M期细胞百分比及细胞 ...
#32. RNA 干涉(RNAi)技术应用于哺乳动物细胞的研究策略
打乱,即“零乱” siRNA (scrambled siRNA), 再对其. 进行BLAST 比对, 防止与目的靶细胞中的其他基. 因有同源性;另一种是在特异性siRNA 中引入几个. 错配碱基。
#33. 雌激素受体α36介导雌激素促进甲状腺乳头状癌细胞增殖的分子 ...
所用PTC细胞株K-1和BCPAP由香港中文大学George G. Chen教授惠赠。 ... 实验前期已构建好ERα36-siRNA干扰质粒,scrambled siRNA作为对照,并成功转 ...
#34. Systemic Delivery of siRNA Specific for Silencing TLR4 Gene ...
The experimental and control groups were treated with 5 [mu]g of TLR4 small interfering RNA (siRNA) or scrambled siRNA. Cardiac histopathology was evaluated ...
#35. LC-MS Analysis of siRNA, Single Guide RNA and Impurities ...
LC-MS Analysis of siRNA, Single Guide RNA and Impurities Using the ... OST Column to chromatographically resolve scrambled oligonucleotide sequences (the ...
#36. The role of Tom70 in cardiac microvascular endothelial cell ...
mol/L, 16h). Then, the expression of Tom70 in MCMECs was knocked down by siRNA, and the HG+HF group was further divided into vehicle group, Scramble siRNA group ...
#37. GAPDH - 中文百科知識
陰性對照(scrambled siRNA)的導入(左)對GAPDH蛋白的表達沒有影響,不過若轉入一個以GAPDH(右)為靶標的siRNA,則會導致GAPDH蛋白表達水平的急劇下降。
#38. Concordance
... expressed relative to the survival of control cells transfected with scrambled siRNA and treated with vehicle; mean ± SD of 3 independent experiments.
#39. Favorable clinical outcome and unique characteristics in ...
Twist1-overexpressing THP1 cells were transiently transfected with either Twist1-targeted siRNA or scrambled control for 48 h, and the nuclear lysates were ...
#40. Dickkopf-1在类风湿关节炎滑膜细胞致炎中的作用
方法将Dkk-1特异性siRNA转染至分离培养的滑膜成纤维细胞( RASF)使Dkk-1基因沉默; ... For Dkk-1 silencing assay, Dkk-1-specific siRNA and control scrambled ...
#41. 探討SPZ1引起人類腎細胞癌化學抗藥性與EGFR活化之關係
以EGFR siRNA 抑制EGFR 表現後處理Cisplatin,發現P-STAT3 及P-ERK 被誘導的趨勢比起Scramble control 有減緩的現象。檢視穩定轉殖細胞株ACHN- SPZ1i (SPZ1 knockdown) ...
#42. Systemic administration of exosomal siRNA inhibited ...
Exosomal siRNA-mediated inhibition of HTT ... PBAs中文培训. EHDN assoc. ... Scramble. RVG-siRNA. Liver. Brain. Lung. In situ hybridization.
#43. RNAi Flashcards - Quizlet
test multiple scrambled or non-targeting negative control molecules. sequence-dependent OTE controls. have multiple siRNA targeting the gene of interest, ...
#44. 脉冲电磁场促进成骨细胞成熟分化依赖于初级纤毛-PI3K/AKT途径
以干扰对照siRNA同时转染对照组ROB,用作干扰对照(scrambled control, SC)组。 1.7. 碱性磷酸酶活性的测定. 成骨细胞用电磁场处理1.5 h后继续培养12 h,测碱 ...
#45. żona skoperta — Translation in English - Maltese - TechDico
Inhibition of egr-1 activation and endothelial cell migration by egr-1 sirna.... (C, D) Confluent HMEC-1s transfected with scrambled siRNA (50 nM) or Egr-1 ...
#46. 小实验,大学问:漫谈对照实验的设计 - 知乎专栏
它可以是一段乱码序列(Scramble control),或者靶向非相关基因(比如靶向GFP、Luciferase等这些在哺乳动物细胞中并不存在的基因)。而如果转染的是装在质粒上的shRNA, ...
#47. 用于有效体内递送dna纳米结构至动脉粥样硬化斑块的材料和方法
使用200nM 的杂乱(scrambled)siRNA对照(ON-TARGETplus Non-targeting, ... <110> 香港中文大学(THE CHINESE UNIVERSITY OF HONG KONG).
#48. 核醣核酸干擾,基因沉默,siRNA | 科學Online - 國立臺灣大學
核糖核酸干擾(RNA interference, RNAi)-下 國立竹北高級中學生物科張雅菱老師/國立台灣師範大學生命科學系李冠群助理教授責任編輯. 請參閱:核糖核酸干擾(RNA ...
#49. Syed Siraj Ahmed Quadri - Lincoln Memorial University
... with NaCl at 130mM (NS), 63mM (LS) or 209mM (HS) alone or in combination with NFAT5 scrambled siRNA, NFAT5 siRNA, or the SIRT-1 inhibitor, EX527.
#50. Histone H3 Antibody | Affinity Biosciences
简体中文 | English | 日本語 ... PTECs treated with LPS, LPS plus GSK, LPS plus DMSO, LPS plus RIP3 siRNA, or LPS plus scrambled siRNA (Scramble) for 12 h.
#51. 厦门大学博硕士论文摘要库 - CORE
中文 摘要. ... 中文摘要. 7. 中文摘要. 肝癌和结肠癌是常见的恶性肿瘤,发现时往往已经到了晚期,因而这两种. 肿瘤都有较高的致死率。 ... Control Scramble SiRNA.
#52. OligoQuest | Bruker
... including tRNA, siRNA, and other modified oligos up to 120 bases, increased the ... Oligonucleotide by-products may include scrambled variants and ...
#53. shHOTAIR对上皮性卵巢癌细胞侵袭及裸鼠致瘤能力的影响
摘要点击次数: 1594. 全文下载次数: 0. 中文摘要: 目的探讨短发夹RNA(shRNA)降低人上皮性卵巢癌SKOV3细胞中长链非编码RNA(lncRNA)HOTAIR基因表达后对SKOV3细胞的 ...
#54. 过度饮酒英文怎么说| 药物依赖性名词-词汇翻译 - SCI论文润色
The vectors did not alter sucrose intake and a scrambled siRNA vector was negative. The data indicate that GABA A α2-regulated TLR4 expression in the CeA ...
#55. 动物用miRNA antagomir案例应用策略解析(20210630)丨含 ...
中文 名:miRNA拮抗剂. 功能:抑制内源性的miRNA ... 动物模型:高脂饮食ApoE-/-小鼠(实验组:注射antagomiR-1;对照组:注射scramble antagomiR).
#56. 1型1-磷酸鞘氨醇受体的siRNA对人涎腺导管上皮细胞的作用
结果:(1)成功构建scramble-siRNA、S1P1-siRNA慢病毒表达载体,慢病毒的滴度约为3.5×108 TU/mL。(2)感染48 h后S1P1-siRNA组HSG细胞中S1P1 mRNA的表达 ...
#57. Horizon Discovery | 進階生物科技股份有限公司
Dharmacon 成立於1995年,現隸屬於Horizon Discovery 的一員。提供高品質的RNAi 系列商品(包含siRNA、shRNA、miRNA 與客製化RNA 服務),以及各物種之cDNA 與ORF 等 ...
scrambled sirna中文 在 2022scrambled control中文-汽車保養配件資訊,精選在PTT ... 的推薦與評價
2022scrambled control中文-汽車保養配件資訊,精選在PTT/MOBILE01討論議題,找scrambled control中文,Scrambled siRNA,scrambled control中文,shrna ... ... <看更多>