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mtagbfp2 在 コバにゃんチャンネル Youtube 的最佳貼文
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Download mTagBFP2.dna file · Fast accurate construct design for all major molecular cloning techniques · Validate sequenced constructs using powerful alignment ... ... <看更多>
mTagBFP2 exhibits 2-fold greater chemical stability and substantially higher brightness in live cells than mTagBFP. mTagBFP2 is also 1.2-fold ... ... <看更多>
#1. mTagBFP2 :: Fluorescent Protein Database
mTagBFP2 is a basic (constitutively fluorescent) blue fluorescent protein published in 2011, derived from Entacmaea quadricolor. It is reported to be a very ...
#2. mTagBFP2 Sequence and Map - SnapGene
Download mTagBFP2.dna file · Fast accurate construct design for all major molecular cloning techniques · Validate sequenced constructs using powerful alignment ...
#3. An enhanced monomeric blue fluorescent protein ... - PubMed
mTagBFP2 exhibits 2-fold greater chemical stability and substantially higher brightness in live cells than mTagBFP. mTagBFP2 is also 1.2-fold ...
#4. pBAD-mTagBFP2 (Plasmid #34632) - Addgene
An enhanced monomeric blue fluorescent protein with the high chemical stability of the chromophore. Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV.
#5. An Enhanced Monomeric Blue Fluorescent Protein with ...
The enhanced photostability and chromophore chemical stability of mTagBFP2 make it a superior protein tag. mTagBFP2 performs well in the ...
#6. FlyBase Experimental Tool Report: mTagBFP2
mTagBFP2 is a monomeric blue fluorescent protein with an excitation peak of 399nm and an emission peak of 454nm. It is an artificial derivative of the ...
#7. Dual-expression system for blue fluorescent protein optimization
(b) Normalized photobleaching curves for mBlueberry2, EBFP2, mTagBFP2, Electra1 and Electra2 expressed in HEK cells (n = 97, 81, 122, 105, 70 ...
#8. FluoTag®-Q anti-TagFP - NanoTag Biotechnologies
Only several years later, in 2011 a monomeric version of TagBFP was described: mTagBFP2. Today more than 800 entries of various fluorescent proteins can be ...
#9. pGG-D-mTagBFP2-E | Gateway Vectors - VIB
Breadcrumb. VIB · VIB-UGent Center for Plant Systems Biology · Gateway Vectors · Collection; pGG-D-mTagBFP2-E ...
#10. Properties of purified mTagBFP2 in comparison with other ...
We finally show that mTagBFP2 can sense exogenously generated nitric oxide when expressed in a living mammalian cell. We propose mTagBFP2 as the starting point ...
#11. H11-CAG-LSL-mtagBFP2 - 南模生物
将CAG-LSL-mTagBFP2-3xFLAG-WPRE-polyA结构定点敲入到小鼠H11位点。该品系小鼠可与Cre小鼠交配,在表达Cre的细胞或组织中表达mTagBFP2-3xFLAG。 应用领域:荧光示踪 ...
#12. mTagBFP2 · Benchling
ACATGCACATGAAGCTGTACATGGAGGGCACCGT TGTACGTGTACTTCGACATGTACCTCCCGTGGCA BanI BtgI MslI MwoI NspI RsaI TatI BsrGI CviQI HpyCH4III mTagBFP2 35 40 45 50 55 60 65.
#13. mTagBFP2 Fused to Human PCNA - Zeiss Campus
The cell culture presented in the digital image in this section is shown expressing a mTagBFP2 fusion tag with PCNA, highlighting replication centers in the ...
#14. The Fluorescent Protein family - Microscopist.co.uk
Points to keep in mind when choosing a fluorescent protein. Key choices for fluorescent proteins. 1. mTagBFP2 (399/454) Brightest blue FP. 2. mCerulean 3 (433 ...
#15. mTagBFP2-Lysosomes-20 vector - Ximbio
Catalogue Number, 157053. Bacterial Resistance, Kan/Neo. Antigen/Gene or Protein Targets, LAMP1 (lgp120). Research Area, Fluorescent Cell Imaging ...
#16. mTagBFP2 - Sharebiology
Fluorescent Proteins. mTagBFP2. Filter fluorescent proteins based on properties. Ex max 399. Em max 454. pKa 2.7. Brightness 32.4. Aggregation. Maturity 12.
#17. CAV mTagBFP2 - PVM - CNRS
CAV mTagBFP2. Description. An E1/E3-deleted, replication-defective, CAV-2 vector harbouring the CMV promoter that leads to a blue fluorescent protein ...
#18. Blue fluorescent protein TagBFP - Evrogen
TagBFP (scientific name mTagBFP) is a monomeric blue fluorescent protein generated by site-specific and random mutagenesis of TagRFP [Subach et al., 2008].
#19. Nitric Oxide Sensing by a Blue Fluorescent Protein - MDPI
We propose mTagBFP2 as the starting point for a new class of genetically encoded nitric oxide sensors based on fluorescence.
#20. Strain: OH15205, Genotype: otEx7057.
Strain: OH15205, Genotype: otEx7057., Description: Pick TagRFP+ animals to maintain. otEx7057 [UPN::NLS::TagRFP-T + acr-5::NLS::mTagBFP2::H2B + ...
#21. Properties of purified mTagBFP2 in comparison ... - Figshare
Properties of purified mTagBFP2 in comparison with other monomeric blue fluorescent proteins.
#22. Vector Biolabs The Adenovirus Co AAV/DJ-CMV-53BP1 ...
Shop Vector Biolabs The Adenovirus Co AAV/DJ-CMV-53BP1-MTAGBFP2 AAV at Fishersci.com.
#23. mTagBFP2TOMM_价格-品牌-详情介绍 - 丁香通
mTagBFP2 -TOMM20 · 价格 $280 · 产地 国产 · 货号 PVT33672 · 品牌 LSM BIO · 技术资料 免费索取技术资料.
#24. Synthetic fluorescent protein expression cassette amp-PLlacO ...
Synthetic fluorescent protein expression cassette amp-PLlacO-mTagBFP2. Taxonomy ID: 1548690 (for references in articles please use NCBI:txid1548690).
#25. lentiGuide-Hygro-mTagBFP2 | Addgene 99374 product information
more info or order · Catalog Number : 99374 · Product Name : lentiGuide-Hygro-mTagBFP2 · article : doi, 10.1016/j. · bacterial resistance : Ampicillin · cloning : ...
#26. Generation of MAFB:mTagBFP2 Reporter iPSC Lines
Generation of MAFB:mTagBFP2 Reporter iPSC Lines. - Targeted iPSC clones were derived from male fibroblast line (ATCC # CRL-.
#27. Jan-Willem Veening on Twitter: "@joachimgoedhart ...
Ideally the blue FP would be compatible with cyan FPs like #mTurquoise2. In our hands #mtagBFP2 is not folding quick enough to be usable." / Twitter ...
#28. mTagBFP2-expressing vectors for electroporation of marine ...
mTagBFP2 -expressing vectors for electroporation of marine protists. Fernan Federici; Isaac Nuñez; Tamara Matute; Peter von Dassow.
#29. d1cc03450b1.pdf - The Royal Society of Chemistry
The mTagBFP2-N-WASP (prey protein) was distributed in cells when the Nck1 protein (bait) was substituted with an HA-tag. Scale bar means 10. µm. Page 20. S20.
#30. pAAV-TREtight-mTagBFP2-B19G - NeuroS - Creative Biolabs
Creative Biolabs offers high-quality pAAV-TREtight-mTagBFP2-B19G to boost neuroscience research.
#31. Phase-separated protein dynamics are ... - microPublication
PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP. (A) Representative live images of ...
#32. ZFIN Engineered Foreign Gene: TagBFP
Citation, Most Citation, Least. Construct, Regulatory Region, Coding Sequence, Species, Tg Lines, Citations. Tg(2xen.cpce-E1B:KALNFB,he1.1:mTagBFP2).
#33. FASTHDR-Cterm-mTagBFP2-Blasticidin plasmid质粒载体
FASTHDR-Cterm-mTagBFP2-Blasticidin plasmid质粒载体-BioVector NTCC典型培养物保藏中心.
#34. Cell-type–specific, multicolor labeling of endogenous ... - PNAS
To test whether split mTagBFP2 functions as a fluorescent probe in S2 ... Labeling of Endogenous Proteins with sfCherry21–10/mTagBFP211 in ...
#35. 1 1. Time Resolved Fluorescence Analysis The decay ... - MDPI
of mTagBFP2, for the different experimental conditions we investigated. Table S1. Results of the deconvolution analysis (amplitudes αi, lifetimes τi) for ...
#36. Figure 5 - Plant Methods
... N. benthamiana leaves with an Agrobacterium pSN.5 p19 culture plus cultures of Agrobacterium containing pSN.5 mTagBFP2 (mTagBFP2), pSN.5 mTFP1 (mTFP1), ...
#37. A toolkit for DNA assembly, genome engineering and ...
A) Emission-based, filter-free images of worms expressing fluorescent proteins from either the Daf-21 (mEGFP and mCherry) or Eft-3 (mTagBFP2 ...
#38. pHBCA11-NLS-mTagBFP2-NLS (7743 bp)
pHBCA11-NLS-mTagBFP2-NLS (7743 bp). AhdI. BsaI. 0009. PciI, BspQI, SapI. AlwNI. PvuII. HindIII, SphI, MfeI. 7000. AmpR. ColEl origin.
#39. Application of an Escherichia coli triple reporter strain for at ...
Here, a triple reporter strain (3RP) was developed by chromosomally integrating the fluorescent proteins mEmerald, CyOFP1, and mTagBFP2 into ...
#40. TagBFP2 Vectors - Regular Plasmid, Lentivirus & AAV
Gene Name TagBFP2; Type ORF; Subtype Fluorescent reporter; Description Also known as mTagBFP2; improved variant of TagBFP generated by I174A mutation ...
#41. 1 Efficient simultaneous mutagenesis of multiple genes in ...
Thirty-two of 86 pSMB(I) and 46 of 88 pSMB(II) T1 seedlings showed both gfp and smb mutant phenotypes, as well as a strong mTagBFP2 signal ...
#42. A Validated Set of Fluorescent-Protein-Based Markers for ...
mTagBFP2 -SKLa. Lipid droplet. Tgl3-GFP. Tgl3-mCherry. Erg6-mCherry. aExcept for those marked, all other constructs are expressed under the ...
#43. Configuring Your Microscope for NeuroPAL | Hobert Lab
mTagBFP2 – a landmark for a subset of neurons, pseudocolored blue. Boundaries: Excitation=300-430nm, Emission=420-470nm(upper bound for GFP/YFP reporters)/445nm ...
#44. Fluorescent Protein Expression Detection - HK
Azurite, TagBFP, mTagBFP, mTagBFP2, Cerulean, ECFP, TagCFP, AmCyan, 383, 400, 400, 400, 433, 439, 458, 458, 450, 456, 456, 456, 475, 476, 480, 489 ...
#45. Phase-separated protein dynamics are ... - USC Dornsife
Figure 1. PGL-1 fluorescently tagged with mKate2 or mTagBFP2 has distinct phase-separation dynamics compared to PGL-1::GFP.
#46. pAAV-TREtight-mTagBFP2-B19G - Identifiers.org
Insert Name: mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G). Organism: Synthetic. Bacterial Resistance: Ampicillin.
#47. US20170260543A1 - Google Patents - Google Patents
mTagBFP2, 399, 454, 50600, 0.64, 32.4, Monomer, 2.7, [48]. Azurite, 383, 450, 26200, 0.55, 14.4, Monomer, 5.0, [28]. EBFP2, 383, 448, 32000, 0.56, 18 ...
#48. Fluorescent Protein Specificity by Nano-Trap
mTagBFP2. n.t.. n.t.. n.t.. n.t.. mCyRFP1. n.t.. n.t.. n.t.. n.t.. TagBFP. -. n.t.. -. ++. SYFP2. n.t.. n.t.. n.t.. n.t.. TagYFP. n.t.. n.t..
#49. Fluorescent Protein Specificity by Antibody
mTagBFP2. n.t.. n.t.. n.t.. n.t.. n.t.. MCYRFP1. n.t.. n.t.. n.t.. n.t.. n.t.. TagBFP. n.t.. n.t.. n.t.. n.t.. n.t.. SYFP2. n.t.. n.t.. n.t..
#50. A Validated Set of Fluorescent-Protein-Based ... - ASM Journals
Some Vps4-mTagBFP2 puncta colocalized with late Golgi/early endosome green markers. (D) A blue marker of peroxisomes. (E) Colocalization between ...
#51. 3M24: Crystal structure of TagBFP fluorescent protein
Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins. · PubMed: 20416505 ...
#52. Evidence for rate-dependent filtering of global extrinsic noise ...
EGFPmVenus. mTagBFP2. mTurquoise2. CitrinemCerulean3. mCherry. mClover3. iRFPmKate2. mMaroon1. mKO2mIFPmScarlet. EGFP. mVenus. mTagBFP2. mTurquoise2.
#53. An Enhanced Monomeric Blue Fluorescent Protein ... - Ebsco
On the basis of mTagBFP we have developed an improved variant, named mTagBFP2. mTagBFP2 exhibits 2-fold greater chemical stability and substantially higher ...
#54. [PDF] Multi-color imaging of the bacterial nucleoid and ...
... TagRFP-T and mRuby2 outperform mCherry as red fluorescent proteins, and that mTagBFP2 can be used as a blue fluorescent protein tag.
#55. A toolbox of stable integration vectors in the fission yeast ...
compatible wavelengths (mTagBFP2, sfGFP or GFP and mCherry), labelling commonly studied cellular organelles, structures and processes.
#56. FPs - I love GFP - Google Sites
name color excitation emission quantum yield brightness (EC*QY/1000) pKa Sirius ultramarine 355 424 0.24 3.6 3.0 below EBFP blue 377 446 0.15 4.5 6.3 SBFP2 blue 380 446 0.47 16 5.5
#57. Solved You cut the pBad-mTagBFP2 plasmid with the BamHI
Question: You cut the pBad-mTagBFP2 plasmid with the BamHI restriction enzyme only. There is only one BamHI RE site. How many bands do you expect to see ...
#58. betway必威官网平台Addgene: pAAV-TREtight-mTagBFP2-B19G
Plasmid pAAV-TREtight-mTagBFP2-B19G from Dr. Ian Wickersham's lab contains the insert mTagBFP2 and rabies virus SAD B19 strain glycoprotein genes (B19G) and ...
#59. Superresolution microscopy of the β-carboxysome reveals a ...
mTagBFP2 -ccmM-mNeonGreen; CA, carbonic anhydrase; CUDA, Compute Unified ... ing a blue fluorescent protein (mTagBFP2) to the N-terminus.
#60. unc-36 - WormBase
Vector for inserting eft-3p:TIR1:F2A:mTagBFP2 transgene by CRISPR/Cas9. From Ashley et al., 2021. wp794. Clone = wp794. | This transgene contains GFP under ...
#61. arXiv:2204.06860v2 [q-bio.BM] 3 Jan 2023
three proteins (GFP, mTagBFP2, mKate2), for different values of number of mutations, M, from the wild type. B: Correlation (Pearson's.
#62. Split-wrmScarlet and split-sfGFP: tools for faster, easier ...
The T2A sequence ensures that mTagBFP2::FP11 and the corresponding mNeonGreen::FP1–10 are separated. The images are representative displays of the ratio of red ...
#63. Servicio de Microscopía Óptica y Confocal (SMOC) Centro de ...
mTagBFP2. Ex/Em: 402/457 nm. Brightness = 33. Monomer. pKa = 2.7. EBFP2. Ex/Em: 383/448 nm. Brightness = 18 Monomer. pKa = 5.3.
#64. Genetically Encoded Ratiometric pH Sensors for ... - ProQuest
The concentration of EGF-CoGFP-mTagBFP2 was determined via the absorbance of the chromophore of mTagBFP2 using ε399 = 56,000 M −1 ·cm −1 [44]. A minor overlap ...
#65. 2)Draw the agarose gel after digesting the plasmid pBAD ...
2)Draw the agarose gel after digesting the plasmid pBAD-mTagBFP2 with following enzymes. a) undigested b) digested with HaeII 3) if the size of insulin gene ...
#66. Chromatic Bacteria – A Broad Host-Range Plasmid ... - Frontiers
The fluorescent protein genes mTagBFP2 (mTB2), mTurquoise2 (mTq2), sGFP2, mClover3 (mCl3), sYFP2, mOrange2 (mO2), mScarlet-I (mSc), and ...
#67. Chromatic Bacteria – A Broad Host-Range Plasmid and ...
The fluorophores mTagBFP2, mTurquoise2, sGFP2, mClover3, sYFP2, mOrange2, mScarlet-I, and mCardinal, encoding for blue, cyan, green, green–yellow, yellow, ...
#68. Constructs - Cas9-triggered homologous recombination
mTagBFP2 is a blue fluorescent protein derived from the same scaffold as TagRFP. This construct was made and deposited at Addgene by Mike Boxem's lab.
#69. mTagBFP2 - WordPress.com
Categoria: mTagBFP2. GST-BFP. Il risultato del lavoro di fusione della GST con la BFP2 estratta dal plasmide mtagBFP2-pBad. A destra il pellet delle cellule ...
#70. Design of Multipartite Transcription Factors for Multiplexed ...
proteins, mTagBFP2, mVenus, mCherry, and iRFP720 (Figure ... fluorescent protein transcript (the fluorescent proteins mTagBFP2, mCitrine, ...
#71. Methods for Synaptic Interrogation - 第 230 頁 - Google 圖書結果
(2017; AAV1-synP-FLEX-splitTVA-EGFP-B19G mixed with AAV1-TREtight-mTagBFP2-B19G) was injected in the somatosensory cortex of PV-Cre × Ai65F (FLPo-dependent ...
#72. Mitochondria Biology - 第 529 頁 - Google 圖書結果
New BFP constructs, such as mTagBFP1 and mTagBFP2, have shifted excitation spectra slightly higher in wavelength to decrease phototoxicity (400nm versus ...
#73. Rapid fluorescent reporter quantification by leaf disc analysis ...
ated the novel bright fluorescent proteins blue mTagBFP2. [31], cyan mTFP1 [32], green mNeonGreen [33], yellow.
#74. Cytek Full Spectrum Viewer
mTagBFP2. Northern Lights™ 493. Northern Lights™ 557. Northern Lights™ 637. NovaFluor Blue 510. NovaFluor Blue 530. NovaFluor Blue 555. NovaFluor Blue 585.
mtagbfp2 在 mTagBFP2 :: Fluorescent Protein Database 的相關結果
mTagBFP2 is a basic (constitutively fluorescent) blue fluorescent protein published in 2011, derived from Entacmaea quadricolor. It is reported to be a very ... ... <看更多>